Cas-CLOVER More Precise Than CRISPR
Cas-CLOVER is unique compared to other targeted gene editing tools as it combines the efficiency of CRISPR/Cas9 with the specificity of TALENs:
High efficiency: Cas-CLOVER is recruited to the target site by guide RNAs (gRNAs) in a similar way as Cas9. However, the Cas9 is completely deactivated “dCas9” and only serves as a linker to our high efficiency proprietary dimeric nuclease Clo51.
High specificity: Cas-CLOVER utilizes two gRNAs which recruit two dCas9-Clo51 nucleases to initiate targeted cleavage. The dCas9-Clo51 nuclease is fully dimeric, meaning activity or cutting only occurs where a pair of nucleases bind correctly to the on-target genomic DNA. This technology has demonstrated best-in-class specificity by deep sequencing and Guide-Seq read-outs.
Easy to Use: The Cas-CLOVER technology can be integrated into any gene editing application or workflow with simple open-source design tools. With clear and independent issued intellectual property, Cas-CLOVER is simple, convenient, and accessible for R&D and commercial applications.