In 2018, Hera BioLabs acquired the rights to the piggyBac transposon system for drug discovery from Transposagen Biopharmaceuticals, where it was used successfully for many years in cell line engineering and transgenic model creation. Hera has adopted the technology for gene editing in cells and offers products & services using this enabling system.
The piggyBac transposon is an efficient non-viral method of stable transfection, inserting transgenes permanently, as well as seamless excision for Footprint-Free® gene editing in combination with Cas-CLOVER or CRISPR nuclease technologies and homologous-recombination. These methods have been demonstrated in over 400 peer-reviewed publications. For more details on the mechanism & applications, please see the piggyBac user manual & the piggyBac technology page.
Key Benefits:
Small to enormous gene integration (200KB+)
Efficiency: Better use of existing resources
High and stable expression
Seamless removal for Footprint-Free gene editing
piggyBac compared to two other transposons. All claims backed by peer-reviewed publications.

1: Rajendra et al. (2016) Generation of Stable Chinese Hamster Ovary Pools Yielding Antibody Titers of up to 7.6 g/L Using the piggyBac Transposon System. Biotechnol. Prog.

2: Rajendra et al. (2017) Bioreactor Scale up and Protein Product Quality Characterization of piggyBac Transposon Derived CHO Pools. Biotechnol. Prog.

3: Rajendra et al. (2017) Evaluation of piggyBac-mediated CHO pools to enable material generation to support GLP toxicology studies. Biotechnol. Prog.

4: Balasubramanian et al. (2018) Generation of high expressing Chinese Hamster Ovary cell pools using the Leap-In transposon system. Biotechnol J. 2018.

5: Balasubramanian et al. (2016) Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines. Biotech & Bioeng.

6: Li et al (2013) piggyBac transposase tools for genome engineering. PNAS.

7: Liu et al (2004) Excision of Sleeping Beauty transposons: parameters and applications to gene therapy. J. of Gene Med.

8: Li et al. (2013) piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice. Disease Model Mechanisms.

9: De Silva et al. (2010) Extending the transposable payload limit of Sleeping Beauty (SB) using the Herpes Simplex Virus (HSV)/SB amplicon vector platform. Gene Therapy.

Ordering & licensing: Hera is the exclusive licensee of this patented technology for drug discovery and early development research and offers the products listed below which can be securely ordered online. Industry customers require the signing of a Limited Use Label License (LULL), which includes an evaluation period of 4 months followed by a research license, fees apply see the LULL. You will be contacted by us regarding the LULL following your order. Academic orders do not require signature but by placing your order you agree to the academic LULL.
Request Reagents or Information on Licensing & Services

Super piggyBac transposase (sPBo) expression vector

Catalog #: SPB-DNA 

Research use only, ask us about commercial licenses

Sequence codon optimized

Storage: ≤ -20C

Estimated delivery is 3-7 business days.

This plasmid is non-renewable & can’t be grown in bacteria

Contact us for bulk orders (>250µg).

Improved Super piggyBac tranpsposase mRNA
Catalog #: SPB-100

Sequence has been codon optimized to provide improved mRNA stability and protein expression in mammalian systems. The piggyBac transposase mRNA is recommended for cells that are not efficiently transfected with vector DNA.

Research use only, ask us about commercial licenses
Release assays: Concentration, Purity, Nucleic Acid Integrity, Potency
Storage: ≤ -60C
Estimated delivery is 3-7 business days.
Contact us for bulk orders (>250µg).

Good Manufacturing Grade super piggyBac transposase mRNA
Catalog #: SPB-200

Sequence has been codon optimized to provide improved mRNA stability and protein expression in mammalian systems. The piggyBac transposase mRNA is recommended for cells that are not efficiently transfected with vector DNA. It is ideally suited for applications where cell lines are to be used for downstream manufacturing, where GMP starting materials are required.

For further manufacturing use
Release assays: Concentration, Purity, Nucleic Acid Integrity, Potency, Residual template DNA, Residual E. coli DNA, Sterility, Endotoxin, B&F, adventitious viruses
Storage: ≤ -60C
Estimated delivery is 3-7 business days.
Contact us for bulk orders (>250µg).

Excision-only piggyBac transposase expression vector
Catalog #: SPB-002

Excision-only piggyBac transposase (PBx) expression vector is a transposase for excision of piggyBac selection cassettes and transgenes for Footprint-Free gene editing or phenotype reversions.

Research Use Only, ask about commercial licensing
Storage <-20C
This vector is non-renewable, and cannot be propagated in bacteria.
Estimated delivery is 3-7 business days.
Contact us for bulk orders (>250µg).

piggyBac transposon base vector
Catalog #: SPB-007

This customizable piggyBac transposon vector contains an insulator-flanked large multiple cloning site (MCS), allowing the flexibility to clone in your promoter and DNA cargo of choice. The core insulators protect cargo from genomic position effects and gene silencing. Sequence available upon request following your order.

Research Use Only
Storage: <-20C
This vector is renewable and can be grown in bacteria
Estimated delivery is 3-7 business days.