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In vitro VCaP cell assay results

VCaP cells were plated onto 96-well plates and allowed 72hours for adhering to plate. Cells were then adjusted to phenol-red-free RPMI 1640 with 5% CSS for another 48 hours. After 7days of incubation with Enz or Bic in agonist or antagonist mode (using synthetic androgen R1881), CellTiter-Glo Luminescent Cell Viability Assay (Promega) was performed using Cytation 3 Cell Imaging Multi-Mode reader (BioTek). Dose-response curves and percent viability of the samples in agonist and antagonist mode were calculated.
Bic effectively induced proliferation of VCaP cells in dose-dependent manner, where as Enz decrease cell viability at higher doses. Both Bic and Enz antagonized the proliferative effect of synthetic androgen R1881.