LOCUS SPB-015 7655 bp DNA circular SYN 18-MAY-2023 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7655) AUTHORS Hera Biolabs TITLE Direct Submission JOURNAL Exported Jan 5, 2024 from SnapGene Viewer 7.1.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..7655 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(239..307) /label=LacZ alpha primer_bind 378..395 /label=M13-fwd protein_bind 432..673 /label=PB-ITR misc_feature 688..919 /label=Core Insulator promoter 928..2106 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongationfactor EF-1-alpha" intron 1159..2097 /label=EF-1-alpha intron A /note="intron upstream of the start codon of humanEF-1-alpha" misc_feature 2123..2192 /label=MCS polyA_signal 2243..2434 /label=SV40 late polyA promoter 2469..3054 /label=PGK Promoter /label=PGK-EM7\Promoter /note="/vntifkey=29" misc_feature 3061..3066 /label=Kozak CDS 3067..3822 /codon_start=1 /label=copGFP /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKM KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL HVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFAR TFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAF KTPIAFARSRAQSSNSAVDGTAGPGSTGSR" CDS 3829..3882 /codon_start=1 /label=T2A /translation="EGRGSLLTCGDVEENPGP" CDS 3889..4485 /codon_start=1 /label=PuroR /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4499..4690 /label=SV40 late polyA misc_feature 4822..5052 /label=Core insulator protein_bind 5064..5376 /label=PB-ITR /label=PB-ITR(1) primer_bind complement(5430..5450) /label=M13-rev misc_binding complement(5456..5478) /label=LacO rep_origin complement(5818..6500) /direction=LEFT /label=ColE1 origin CDS complement(6598..7257) /label=AmpR ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggagatcggt acttcgcgaa 421 tgcgtcgaga tttaacccta gaaagataat catattgtga cgtacgttaa agataatcat 481 gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga 541 atagatatta agttttatta tatttacact tacatactaa taataaattc aacaaacaat 601 ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct tctataaagt 661 aacaaaactt ttatagaatt ctatctagag ggacagcccc cccccaaagc ccccagggat 721 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt 781 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa 841 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc 901 tggggggata cggggaaaag gcctccaggc tccggtgccc gtcagtgggc agagcgcaca 961 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg agcctagaga 1021 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 1081 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 1141 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg 1201 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat 1261 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta acgagcccct 1321 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg 1381 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga 1441 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg 1501 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc 1561 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct 1621 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg 1681 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc 1741 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca 1801 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag 1861 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta 1921 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa 1981 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga 2041 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg 2101 tcgtgaggta cgggatccac cgcttaagcg ctgatcaatt ggcgcgccga attcgttaac 2161 aagctttaat taacgcgtat accgaaggta ccttcgtagc gcttacctca gcacaagcta 2221 cgcgtatatg tcctcacagg aacagacatg ataagataca ttgatgagtt tggacaaacc 2281 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 2341 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 2401 tttcaggttc agggggaggt gtgggaggtt ttttcgaagt ccctaaagaa acagttaatc 2461 gcgatcgcaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc 2521 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc 2581 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc 2641 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg 2701 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg 2761 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc 2821 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc 2881 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc 2941 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcctgt tgacaattaa 3001 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccagatct 3061 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc 3121 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag 3181 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg 3241 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag 3301 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac 3361 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc 3421 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag 3481 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg 3541 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac 3601 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg 3661 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac 3721 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct 3781 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgtcgacga gggcagagga 3841 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctggatccat gaccgagtac 3901 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtccg caccctcgcc 3961 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 4021 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 4081 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 4141 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 4201 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 4261 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 4321 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 4381 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 4441 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt cgacagggca 4501 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa 4561 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat 4621 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg 4681 gaggtttttt gccccggaat tcctaggatc cggccggcct gcaggtgtcc tcacaggaac 4741 gaagtcccta aagaaacagt ggcagccagg tttagccccg gaattgactg gattcctttt 4801 ttagggccca ttggtatggc tttttccccg tatcccccca ggtgtctgca ggctcaaaga 4861 gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc 4921 cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc 4981 ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg gctttggggg 5041 ggggctgtcc cttagcggcc gctgatatct ataacaagaa aatatatata taataagtta 5101 tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc 5161 acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg 5221 acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct 5281 aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat 5341 gcgtcaattt tacgcagact atctttctag ggttaaatcg gatgccggga ccgacgagtg 5401 cagaggcgtg caagcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 5461 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 5521 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 5581 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 5641 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 5701 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 5761 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 5821 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 5881 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 5941 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 6001 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 6061 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 6121 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 6181 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 6241 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 6301 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 6361 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 6421 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 6481 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 6541 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 6601 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 6661 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 6721 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 6781 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 6841 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 6901 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 6961 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 7021 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 7081 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 7141 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 7201 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 7261 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 7321 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 7381 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 7441 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 7501 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 7561 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 7621 ctataaaaat aggcgtatca cgaggccctt tcgtc //