LOCUS SPB-014 7174 bp DNA circular SYN 18-MAY-2023 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7174) AUTHORS Hera Biolabs TITLE Direct Submission JOURNAL Exported Jan 5, 2024 from SnapGene Viewer 7.1.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..7174 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(239..307) /label=LacZ alpha primer_bind 378..395 /label=M13-fwd protein_bind 432..673 /label=PB-ITR misc_feature 688..919 /label=Core Insulator promoter 1065..1650 /label=PGK Promoter /label=PGK-EM7\Promoter /note="/vntifkey=29" misc_feature 1661..1744 /label=MCS polyA_signal 1762..1953 /label=SV40 late polyA promoter 1988..2573 /label=PGK Promoter /label=PGK-EM7\Promoter /note="/vntifkey=29" misc_feature 2580..2585 /label=Kozak CDS 2586..3341 /codon_start=1 /label=copGFP /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKM KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL HVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFAR TFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAF KTPIAFARSRAQSSNSAVDGTAGPGSTGSR" CDS 3348..3401 /codon_start=1 /label=T2A /translation="EGRGSLLTCGDVEENPGP" CDS 3408..4004 /codon_start=1 /label=PuroR /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4018..4209 /label=SV40 late polyA misc_feature 4341..4571 /label=Core insulator protein_bind 4583..4895 /label=PB-ITR /label=PB-ITR(1) primer_bind complement(4949..4969) /label=M13-rev misc_binding complement(4975..4997) /label=LacO rep_origin complement(5337..6019) /direction=LEFT /label=ColE1 origin CDS complement(6117..6776) /label=AmpR ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggagatcggt acttcgcgaa 421 tgcgtcgaga tttaacccta gaaagataat catattgtga cgtacgttaa agataatcat 481 gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga 541 atagatatta agttttatta tatttacact tacatactaa taataaattc aacaaacaat 601 ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct tctataaagt 661 aacaaaactt ttatagaatt ctatctagag ggacagcccc cccccaaagc ccccagggat 721 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt 781 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa 841 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc 901 tggggggata cggggaaaag gcctccaagg ccagcttccc acaataagtt gggtgaattt 961 tggctcattc ctcctttcta taggattgag gtcagagctt tgtgatggga attctgtgga 1021 atgtgtgtca gttagggtgt ggaaagtccc gcgatcgcct aggtaattct accgggtagg 1081 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 1141 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 1201 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 1261 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 1321 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 1381 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 1441 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 1501 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 1561 tccgggcctt tcgacctgca gcctgttgac aattaatcat cggcatagta tatcggcata 1621 gtataatacg acaaggtgag gaactaaacc agatctgcca gtttaaacaa gcctcaggtc 1681 cccgaccggt cattggcgcg ccgaaggtac cttcgtagcg cttacctcag cacaagctac 1741 gcgtatatgt cctcacagga acagacatga taagatacat tgatgagttt ggacaaacca 1801 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat 1861 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt 1921 ttcaggttca gggggaggtg tgggaggttt tttcgaagtc cctaaagaaa cagttaatcg 1981 cgatcgcaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct 2041 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc 2101 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct 2161 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga 2221 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg 2281 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct 2341 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg 2401 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc 2461 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcctgtt gacaattaat 2521 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accagatctg 2581 ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg 2641 gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccaagcagg 2701 gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc 2761 tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga 2821 accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg 2881 aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg 2941 gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga 3001 tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac gtgctggtgg 3061 gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc gtggtggaca 3121 gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt 3181 tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc gtggagtacc 3241 agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg tccaattctg 3301 ccgtggacgg caccgccgga cccggctcca ccggatctcg cgtcgacgag ggcagaggaa 3361 gtcttctaac atgcggtgac gtggaggaga atcccggccc tggatccatg accgagtaca 3421 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtccgc accctcgccg 3481 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc 3541 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 3601 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 3661 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 3721 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 3781 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 3841 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc 3901 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc 3961 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtc gacagggcag 4021 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat 4081 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata 4141 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg 4201 aggttttttg ccccggaatt cctaggatcc ggccggcctg caggtgtcct cacaggaacg 4261 aagtccctaa agaaacagtg gcagccaggt ttagccccgg aattgactgg attccttttt 4321 tagggcccat tggtatggct ttttccccgt atccccccag gtgtctgcag gctcaaagag 4381 cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc 4441 ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg 4501 gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg 4561 gggctgtccc ttagcggccg ctgatatcta taacaagaaa atatatatat aataagttat 4621 cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca 4681 cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga 4741 cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta 4801 aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg 4861 cgtcaatttt acgcagacta tctttctagg gttaaatcgg atgccgggac cgacgagtgc 4921 agaggcgtgc aagcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 4981 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 5041 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 5101 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 5161 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 5221 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 5281 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 5341 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 5401 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 5461 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 5521 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 5581 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 5641 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 5701 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 5761 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 5821 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 5881 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 5941 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 6001 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 6061 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 6121 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 6181 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 6241 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 6301 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 6361 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 6421 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 6481 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 6541 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 6601 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 6661 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 6721 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 6781 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 6841 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 6901 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 6961 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 7021 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 7081 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 7141 tataaaaata ggcgtatcac gaggcccttt cgtc //