LOCUS SPB-013 8377 bp DNA circular SYN 18-MAY-2023 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8377) AUTHORS Hera Biolabs TITLE Direct Submission JOURNAL Exported Jan 5, 2024 from SnapGene Viewer 7.1.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..8377 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(239..307) /label=LacZ alpha primer_bind 378..395 /label=M13-fwd protein_bind 432..673 /label=PB-ITR misc_feature 688..919 /label=Core Insulator enhancer 1123..1502 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1130..2840 /label=CAG /label=CAGGS /note="CAG Promoter 1592..3302" /note="/ApEinfo_fwdcolor=#00ffff" /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /note="/ApEinfo_revcolor=#00ffff" promoter 1504..1776 /label=chicken beta-actin promoter intron 1777..2789 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" misc_feature 2861..2944 /label=MCS polyA_signal 2965..3156 /label=SV40 late polyA promoter 3191..3776 /label=PGK Promoter /label=PGK-EM7\Promoter /note="/vntifkey=29" misc_feature 3783..3788 /label=Kozak CDS 3789..4544 /codon_start=1 /label=copGFP /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKM KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL HVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFAR TFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAF KTPIAFARSRAQSSNSAVDGTAGPGSTGSR" CDS 4551..4604 /codon_start=1 /label=T2A /translation="EGRGSLLTCGDVEENPGP" CDS 4611..5207 /codon_start=1 /label=PuroR /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 5221..5412 /label=SV40 late polyA misc_feature 5544..5774 /label=Core insulator protein_bind 5786..6098 /label=PB-ITR /label=PB-ITR(1) primer_bind complement(6152..6172) /label=M13-rev misc_binding complement(6178..6200) /label=LacO rep_origin complement(6540..7222) /direction=LEFT /label=ColE1 origin CDS complement(7320..7979) /label=AmpR ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggagatcggt acttcgcgaa 421 tgcgtcgaga tttaacccta gaaagataat catattgtga cgtacgttaa agataatcat 481 gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga 541 atagatatta agttttatta tatttacact tacatactaa taataaattc aacaaacaat 601 ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct tctataaagt 661 aacaaaactt ttatagaatt ctatctagag ggacagcccc cccccaaagc ccccagggat 721 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt 781 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa 841 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc 901 tggggggata cggggaaaag gcctccaagg ccagcttccc acaataagtt gggtgaattt 961 tggctcattc ctcctttcta taggattgag gtcagagctt tgtgatggga attctgtgga 1021 atgtgtgtca gttagggtgt ggaaagtccc gcgatcgcta gcttaagcgc tgatcaattg 1081 gcgcgccgaa ttcgttaaca agctttaatt aacctgcagg tcgacattga ttattgacta 1141 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 1201 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 1261 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 1321 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 1381 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 1441 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 1501 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc 1561 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg 1621 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt 1681 gcggcggcag ccaatcagag cggcgcgctc cgaaatcctt ttatggcgag gcggcggcgg 1741 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc 1801 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc 1861 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa 1921 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct 1981 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc 2041 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg 2101 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg 2161 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg 2221 cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc 2281 cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg 2341 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg 2401 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc 2461 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg 2521 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt 2581 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc 2641 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac 2701 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc 2761 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt 2821 ctcatcattt tggcaaagaa ttcgatagtg gaatggtgga gtttaaacaa gcctcaggtc 2881 cccgaccggt cattggcgcg ccgaaggtac cttcgtagcg cttacctcag cacaagctac 2941 gcgtattata tgtcctcaca ggaacagaca tgataagata cattgatgag tttggacaaa 3001 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt 3061 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta 3121 tgtttcaggt tcagggggag gtgtgggagg ttttttcgaa gtccctaaag aaacagttaa 3181 tcgcgatcgc aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc 3241 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat 3301 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct 3361 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg 3421 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa 3481 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg 3541 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg 3601 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct 3661 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagcct gttgacaatt 3721 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccagat 3781 ctgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca 3841 ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc 3901 agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc 3961 tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg 4021 agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt 4081 acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga 4141 tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca 4201 agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg 4261 tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg 4321 acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca 4381 tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt 4441 accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt 4501 ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgtcgac gagggcagag 4561 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctggatcc atgaccgagt 4621 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgtc cgcaccctcg 4681 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 4741 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 4801 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 4861 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 4921 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 4981 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 5041 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 5101 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 5161 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaggg 5221 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 5281 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 5341 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt 5401 gggaggtttt ttgccccgga attcctagga tccggccggc ctgcaggtgt cctcacagga 5461 acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt 5521 ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa 5581 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg 5641 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg 5701 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg 5761 ggggggctgt cccttagcgg ccgctgatat ctataacaag aaaatatata tataataagt 5821 tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag 5881 tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag 5941 tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc 6001 ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc 6061 atgcgtcaat tttacgcaga ctatctttct agggttaaat cggatgccgg gaccgacgag 6121 tgcagaggcg tgcaagcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 6181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 6241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 6301 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 6361 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 6421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 6481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 6541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 6601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 6661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 6721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 6781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 6841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 6901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 6961 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 7021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 7081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 7141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 7201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 7261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 7321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 7381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 7441 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 7501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 7561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 7621 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 7681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 7741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 7801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 7861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 7921 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 7981 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 8041 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 8101 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 8161 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 8221 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 8281 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 8341 acctataaaa ataggcgtat cacgaggccc tttcgtc //