LOCUS SPB-012 7182 bp DNA circular SYN 18-MAY-2023 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7182) AUTHORS Hera Biolabs TITLE Direct Submission JOURNAL Exported Jan 5, 2024 from SnapGene Viewer 7.1.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..7182 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(239..307) /label=LacZ alpha primer_bind 378..395 /label=M13-fwd protein_bind 432..673 /label=PB-ITR misc_feature 688..919 /label=Core Insulator enhancer 1072..1451 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1452..1655 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate earlypromoter" misc_feature 1669..1752 /label=MCS polyA_signal 1770..1961 /label=SV40 late polyA promoter 1996..2581 /label=PGK Promoter /label=PGK-EM7\Promoter /note="/vntifkey=29" misc_feature 2588..2593 /label=Kozak CDS 2594..3349 /codon_start=1 /label=copGFP /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKM KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL HVSFSYRYEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFAR TFSLRDGGYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAF KTPIAFARSRAQSSNSAVDGTAGPGSTGSR" CDS 3356..3409 /codon_start=1 /label=T2A /translation="EGRGSLLTCGDVEENPGP" CDS 3416..4012 /codon_start=1 /label=PuroR /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4026..4217 /label=SV40 late polyA misc_feature 4349..4579 /label=Core insulator protein_bind 4591..4903 /label=PB-ITR /label=PB-ITR(1) primer_bind complement(4957..4977) /label=M13-rev misc_binding complement(4983..5005) /label=LacO rep_origin complement(5345..6027) /direction=LEFT /label=ColE1 origin CDS complement(6125..6784) /label=AmpR ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggagatcggt acttcgcgaa 421 tgcgtcgaga tttaacccta gaaagataat catattgtga cgtacgttaa agataatcat 481 gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga 541 atagatatta agttttatta tatttacact tacatactaa taataaattc aacaaacaat 601 ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct tctataaagt 661 aacaaaactt ttatagaatt ctatctagag ggacagcccc cccccaaagc ccccagggat 721 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt 781 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa 841 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc 901 tggggggata cggggaaaag gcctccaagg ccagcttccc acaataagtt gggtgaattt 961 tggctcattc ctcctttcta taggattgag gtcagagctt tgtgatggga attctgtgga 1021 atgtgtgtca gttagggtgt ggaaagtccc gcgatcgcta gcttaagagc tgacattgat 1081 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 1141 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 1201 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 1261 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 1321 atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 1381 cccagtacat gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg 1441 ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact 1501 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 1561 atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta 1621 ggcgtgtacg gtgggaggtc tatataagca gagctgtgga atggtggagt ttaaacaagc 1681 ctcaggtccc cgaccggtca ttggcgcgcc gaaggtacct tcgtagcgct tacctcagca 1741 caagctacgc gtatatgtcc tcacaggaac agacatgata agatacattg atgagtttgg 1801 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 1861 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 1921 ttttatgttt caggttcagg gggaggtgtg ggaggttttt tcgaagtccc taaagaaaca 1981 gttaatcgcg atcgcaattc taccgggtag gggaggcgct tttcccaagg cagtctggag 2041 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca 2101 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc 2161 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca 2221 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg 2281 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt 2341 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg 2401 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca 2461 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcctgttga 2521 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac 2581 cagatctgcc accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg 2641 catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc 2701 caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc 2761 ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg 2821 ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga 2881 gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg 2941 cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac 3001 cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt 3061 gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt 3121 ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg 3181 ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt 3241 ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc 3301 caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgcg tcgacgaggg 3361 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggccctg gatccatgac 3421 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtccgcac 3481 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca 3541 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 3601 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 3661 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 3721 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 3781 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 3841 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 3901 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 3961 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagtcga 4021 cagggcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 4081 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 4141 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 4201 ggtgtgggag gttttttgcc ccggaattcc taggatccgg ccggcctgca ggtgtcctca 4261 caggaacgaa gtccctaaag aaacagtggc agccaggttt agccccggaa ttgactggat 4321 tcctttttta gggcccattg gtatggcttt ttccccgtat ccccccaggt gtctgcaggc 4381 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg 4441 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc 4501 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct 4561 ttgggggggg gctgtccctt agcggccgct gatatctata acaagaaaat atatatataa 4621 taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt 4681 aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa 4741 atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag 4801 atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag 4861 aatgcatgcg tcaattttac gcagactatc tttctagggt taaatcggat gccgggaccg 4921 acgagtgcag aggcgtgcaa gcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt 4981 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 5041 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 5101 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 5161 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 5221 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 5281 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 5341 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 5401 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 5461 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 5521 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 5581 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 5641 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 5701 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 5761 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 5821 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 5881 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 5941 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 6001 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 6061 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 6121 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 6181 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 6241 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 6301 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 6361 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 6421 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 6481 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 6541 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 6601 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 6661 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 6721 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 6781 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 6841 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 6901 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 6961 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 7021 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 7081 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 7141 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc //