Immunodeficient mice engrafted with CD34+ hematopoietic stem/progenitor cells (HSPCs) are an attractive humanized mouse model for immuno-oncology efficacy studies, as they are a validated platform that provides bone marrow engraftment with development of multi-lineage human immune cells in the peripheral blood.
For many studies, such as those with engineered cells (e.g. HSPCs and CAR-T), it may be preferential that the study sponsor dictates the donor for CD34+ humanized mice. Hera has demonstrated through effective recipient conditioning, transplantation and flow cytometric (FC) analysis efficient engraftment of engineered CD34+ HSPCs as indicated by high levels of human CD45+ chimerism in the blood and bone marrow. We offer custom donor as well as standardized donor humanized mouse studies for preclinical immuno-oncology.
Immuno-Oncology Services
Our team of experienced scientists provide immuno-oncology services utilizing humanized immune system mouse models with CD34+ cells. Humanized mouse models are designed to closely mimic the human immune system, enabling us to evaluate the efficacy and safety of novel immuno-oncology therapies in a preclinical setting.
- Immune cell profiling and characterization
- In vivo tumor growth and tumor infiltration
- Efficacy and toxicity studies of immuno-oncology therapies
- Mechanistic studies of immune response to cancer
![Human Immune Cell Engraftment humanized mice](http://herabiolabs.com/storage/2023/03/Human-Immune-Cell-Engraftment.jpg)
Advantages for Immuno-Oncology Research
Mice with a humanized immune system offers several advantages over immunodeficient preclinical models, including:
- Replication of human immune responses and tumor immune cell infiltration.
- Ability to evaluate drug efficacy and toxicity in the context of a human immune system.
- More reliable prediction of clinical outcomes in humans.
At Hera, we are committed to advancing Immuno-Oncology research and helping our clients achieve their goals. Contact us today to learn more about our services and how we can help accelerate your research.
![SRG rat](http://herabiolabs.com/storage/2020/09/rat-300x201.jpg)
Humanized Immune System in Mice
FC analysis of peripheral blood for human CD45+ at 8 weeks.
![Chimeric huIs Mouse chimeric custom donor engraftment in NSG mice](http://herabiolabs.com/storage/2019/03/blood.png)
Blood collected at 8 weeks post-transplant of G-CSF mobilized peripheral blood CD34+ cells was subjected to FC analysis for human and mouse CD45. Left panel: group 1 (vehicle control); center two panels: group 2; right two panels: group 3.
FC analysis of bone marrow for human CD45+ at 8 weeks.
![Chimeric huIs Mouse Blone Marrow Analaysis Chimeric humanized mouse bone marrow](http://herabiolabs.com/storage/2019/03/bone-marrow.png)
Bone marrow collected at 8 weeks post-transplant of G-CSF mobilized peripheral blood CD34+ cells was subjected to FC analysis for human and mouse CD45. Left panel: group 1 (vehicle control); center two panels: group 2; right two panels: group 3.
![hera icons-drug-dev](http://herabiolabs.com/storage/2021/02/hera-icons-drug-dev-300x300.png)