GFP+ SH-SY5Y Cell Line
SH-SY5Y is a human neuroblastoma cell line widely used in systematic toxicological assays1, high-throughput screenings, and in the study of CNS disorders such as Alzheimer’s or Parkinson’s diseases2. SH-SY5Y cells can be differentiated into mature neurons at a high efficiency and utilized for screening and assays3. To further increase their utility, Hera has modified the neuroblastoma cell line using the (non-viral) piggyBac transposon technology to stably express green fluorescent protein (GFP). The stable GFP+ SH-SY5Y cell line retains the same morphology and the ability to differentiate as the unmodified SH-SY5Y cell line.
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- Breier J, Gassmann K, Kayser R, et al. Neural progenitor cells as models for high-throughput screens of developmental neurotoxicity: State of the science. Neurotoxicology and teratology. 2009;32(1):4–15.
- Loh SHY, Francescut L, Lingor P, Bähr M, Nicotera P. Cell death and differentiation – abstract of article: Identification of new kinase clusters required for neurite outgrowth and retraction by a loss-of-function RNA interference screen. Cell Death & Differentiation. 2007;15(2):283–298. doi:10.1038/sj.cdd.4402258