Hera to present at the 2015 American College of Toxicology “GFP+ HUMAN STEM CELL DERIVED NEURONS AMENABLE TO HIGH CONTENT NEURITE OUTGROWTH AND HIGH THROUGHPUT TOXICOLOGY ASSAYS”.
High throughput (HTS) and high content screening (HCS) assays often utilize cell sources, which are not amenable to large-scale screening and can require extensive cell culture and processing prior to imaging. To overcome this hurdle, Hera and a commercial partner ArunA Biomedical, engineered human neural progenitors derived from stem cells with a non-viral vector encoding a GFP reporter gene using a selectable piggyBac™ gene editing system to produce hNP1 GFP+ cells. hNP1 reporter cells exhibit detectable dose dependent response to toxins tested, (cytotoxicity and cell migration) providing a sensitive, high throughput and high content amenable, cell based human neurotox assay platform.
download Hera’s ACT 2015 Poster