Cancer Xenografts in Mice & Rats

Cancer cell lines or patient derived xenograft (PDX) models are commonly engrafted and screened in mouse strains such as NSG®, nude, and NOD-SCID mice. However, rat models may provide certain advantages for cancer xenograft studies, such as larger tissue/blood samples and typically more translational compound PK properties, but a lack in severely immunodeficient rat models has limited their use. In order to overcome the limitations of current immunodeficient rat and mouse models, Hera has introduced several SCID rat strains, Rag2 knockout and a Rag2/Il2rg double knockout, the SRG™ rat, which are more immunodeficient than Nude rats and amenable for xenotransplantation.  Hera provides SCID models to researchers off-the-shelf as well as tumor xenograft efficacy studies conducted at Hera.

Hera has created a Rag2 knockout and a Rag2/Il2rg double knockout, the SRG™ Rat. The SCID rat models provide research opportunities not available in current mouse and rat models
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Growth rate of H358 xenografts in SDR™ (Hera) rats compared to NSG and Nude mice. 1, 5, and 10 million cells where injected subcutaneously into Rag2 KO rats and tumor volume measured.

Leukemia blasts present in tumors resulting from  IP injection of a leukemia cell line in Rag2 knockout rats.  

Efficacy Study Services

  • Xenograft/PDX implantation and efficacy screening services
  • PDX model creation and validation
  • Gene editing (reporters cell lines for in-vivo imaging or disease models)
  • Off-the-shelf rodent models

Xenograft Cell Lines and PDX

Efficacy studies can be conducted with xenograft cell lines or patient derived tumor samples either chosen from commercially available options or client provided cells.

Customized Study Design

Almost all details of the efficacy study can be customized to meet your needs.  From the length and size of the study to special and unique request.  Our team of expert scientists can help design and implement the ideal study for you.

In additional to flexible study design, additional measurements and data can be added to your study, including  histology, ophthalmology, toxicokinetic, necropsy, histopathology, and clinical pathology and more.

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Basic Study Outline
Model Species:SRG rats (SD-Rag2-Il2rg KO)
NOG/NSG/NCG mice etc.
Length:28 days
Study Design:3 test article treated groups,  low dose, mid dose and high dose,  and 2 controls. 5 males and 5 females for each group.
Dosing Method:Oral gavage, daily
Tumor Implantation:Subcutaneous (subQ) or orthotopic
Observations:Tumor size, bodyweight, food consumption, and clinical observations.
Reporting:Raw Data, Draft, and Final Report