Cancer Cell Line & PDX Services
Cancer cell lines or patient derived xenograft (PDX) models are commonly engrafted and screened in mouse models of cancer such as NSG mice, nude mice, and SCID mice. However, rats may provide certain advantages as a cancer xenograft model, such as larger tissue/blood samples and typically more translational compound PK properties. Unfortunately, a lack in severely immunodeficient rat models has limited their use, until now. For example, the athymic Nude rat or RNU rat is only partially immunodeficient lacking T-cells. In order to overcome the limitations of current immunodeficient rat and mouse models, Hera has introduced a Rag2/Il2rg double knockout SCID rat, the SRG rat™, which are more immunodeficient than Nude rats and amenable as tumor models and for humanization. Hera provides the SRG rat to researchers off-the-shelf as well as tumor xenograft studies conducted at Hera.
Efficacy Study Services
PDX-derived cell line grows ~10x bigger in SRG rat vs NSG mouse
When a cell line derived from an ovarian PDX model was transplanted SQ the take rate was 100% in both species but the tumor ten-fold bigger in the SRG rat compared with the NSG mouse, in the same amount of time. The SRG rat thus provides the possibility of getting adequate sample size from PDX tissue for an efficacy study faster than mouse. Download AACR Poster on PDX Models for more data.
VCaP tumor growth in SRG rats
VCaP prostate cancer cells known to have poor engraftment rates and kinetics in immunodeficient mice (i.e. nude mice, SCID mice, NSG mice), thrived in the SRG rat. Preclinical studies with Enzalutamide against the VCaP tumor performed as expected. Download AACR Poster on Humanization.
Humanized mice engrafted with components of the human immune system enable preclinical animal studies for therapeutic immune modulation, tumor-immune system interactions and tumor immune escape. Humanized mice are often desired over or complement syngeneic mouse models which use the mouse immune system. Hera has demonstrated capabilities in humanized NOD SCID mouse and SRG rats for preclinical oncology services. All of Hera’s cancer cell line and PDX model services can be conducted in immunodeficient mice or rats as well as humanized mice or rats. Please see our Immuno-oncology in Humanized Rodents page for demonstration data and information.
Xenograft Cell Lines and PDX
Customized Study Design
In additional to flexible study design, additional measurements and data can be added to your study, including histology, ophthalmology, toxicokinetic, necropsy, histopathology, and clinical pathology and more.
|Basic Study Outline|
|Model Species:||SRG rats (SD-Rag2-Il2rg KO)|
NSG mice, Nude mice, SCID mice, NOG mice, NCG mice, humanized mice or SRG rats etc.
|Study Design:||3 test article treated groups, low dose, mid dose and high dose, and 2 controls. 5 males and 5 females for each group.|
|Dosing Method:||Oral gavage, daily|
|Tumor Implantation:||Subcutaneous (subQ) or orthotopic mouse model or rat model|
|Observations:||Tumor size, bodyweight, food consumption, and clinical observations.|
|Reporting:||Raw Data, Draft, and Final Report|